鸡大肠杆菌(O_(78))超声波灭活菌苗研究

The Immunogenicity of Ultrasonicated Vaccine of Chicken Pathogenic E.coli (O 78 ) Against Colibacillosis

菌毛提取液、荚膜多糖－破伤风类毒素（ＣＰＳ－ＴＴ）载体抗原液及热、甲醛和超声波灭活菌液中加入蜂胶佐剂，制成菌苗免疫石岐杂鸡，７７及１１２ｄ攻毒保护试验结果表明，超声波灭活菌体的免疫原性最好，热灭活菌体最差，菌毛、ＣＰＳ－ＴＴ及甲醛灭活菌体的免疫原性相当间接血凝试验（ＩＨＡ）较微量凝集试验对抗大肠杆菌（Ｅ．ｃｏｌｉ）抗体更敏感。

The pili extract,capsular polysaccharide tetanus toxoid (CPS-TT) and heat ,formaldehyde and ultrasound inactivated bacteria were mixed with propolis adjuvant respectively to make five vaccines.Challenge experiments at 21 days post first injection and 40 days post second vaccination revealed that ultrasonic inactivation followed by irradiation was the most efficient method for the preparation of an effective anticolibacillosis vaccine.Formaldehyde inactivated bacteria、the pili and the CPS TT vaccines provided similar protection but were less protective.Indirect hemagglutination test was much more sensitive in detecting E.coli antibodies than the micro agglutination test( P <0 01),especially when used on ultrasonicated antigen.