人工合成柞蚕抗菌肽D基因转化沙田柚

Transformation of the Shatian Pummelo ( Citrus grandis) with the Synthetic Oak Silkworm Antibacterial Peptide D Gene

采用根癌农杆菌介导法将柞蚕抗菌肽Ｄ基因导入沙田柚上胚轴等外植体，经卡那霉素筛选，获得了若干转基因植株对这些植株进行胭脂碱电泳检测、点印迹杂交、ＰＣＲ扩增和Ｓｏｕｔｈｅｒｎ杂交分析。

Some transgenic plantlets of Shatian pummelo, Citrus grandis were obtained by infection with Agrobacterium tumefaciens which contained the NPTⅡ gene, nos gene, CaMV 35s promoter and Ap-D gene . The infected tissues and buds were selected on MS medium containing different concentration of Km as necessary. The transformants were selected on the basis of Km resistance (Km r), nopaline electrophoretic detection, DNA dot blotting, PCR amplification and Southern hybridization. The results showed that the Antibacterial peptide D gene had been integrated into the genome of transgenic pummelo plant cells.