腺胃病变型鸡传染性支气管炎病毒的分离鉴定

Isolation and identification of a Proventriculus Disease Avian Infectious Bronchitis Virus

从１９９７年在辽宁省大连市某鸡场发生的一种以腺胃肿大为特征的鸡的传染病病例中收集腺胃组织（Ｄ９７１）接种ＳＰＦ鸡胚，传至１３代，具有规律性死亡和典型胚胎病变特征，ＥＩＤ５０为１０－６．４８／０．２ｍｌ。Ｄ９７１毒株感染ＳＰＦ鸡胚尿囊液经负染电镜观察，可见直径为８０～１２０ｎｍ，有囊膜和纤突的冠状病毒粒子。Ｄ９７１毒株经卵磷脂酶Ｃ处理后能凝集鸡红细胞，并能特异的被Ｄ９７１多抗所抑制。用反转录聚合酶链反应获得了Ｄ９７１毒株免原（Ｓ１）基因ｃＤＮＡ，经１％琼脂糖凝胶电泳可见１．７ｋｂ的特征性条带。用Ｄ９７１毒株接种ＳＰＦ鸡，能引起与自然病例相似的病理变化，并分离到病毒。用Ｄ９７１毒株可直接感染ＳＰＦ鸡胚成纤维细胞。结果证实从腺胃病变型ＩＢ鸡群中分离的Ｄ９７１毒株是冠状病毒科ＩＢＶ成员。

One Local strain(designaed as D971)of Avian Infectious Bronchitis Virus (IBV),which caused a new type proventriculus disease in the field,was isolated from a chicken farm in Dalian city It was identified as IBV by virus morphology,hemagglutination activity,pathology on chickens and chicken embryos,cell culture and the amplifidcation of S 1 gene using RT PCR.Results demonstrated that the present isolate was a new type virus of IBV.