树兰未成熟种子离体快繁技术

Rapid Propagation of Immature Seeds of Tree Orchid(Epidendrum schbomburgkii) in vitro

以树兰未成熟种子为外植体,研究了原球茎的诱导、增殖、萌芽及生根培养等几个关键步骤的配方。结果表明:树兰种子播种在MS+BA0.5mg·L-1+NAA0.25mg·L-1+蔗糖25g·L-1培养基上,7～10d后开始有绿点产生,25d左右就可看见原球茎团;1/2MS+6-BA1.5mg·L-1+NAA0.25mg·L-1+蔗糖20g·L-1+CH2.0g·L-1的培养基对原球茎增殖的效果最佳,增殖倍数超过9倍;将原球茎转入1/2MS+NAA0.1mg·L-1+6-BA0.2mg·L-1+蔗糖25g·L-1的培养基上,原球茎的出芽率超过90%;将芽接种在1/2MS+NAA0.5mg·L-1+BA0.1mg·L-1+香蕉泥70g·L-1+蔗糖25g·L-1的生根培养基中,生根率达94%;以水苔作为移栽基质较理想,移栽成活率可达92.5%,且植株生长健壮。

The immature seeds of tree orchid （Epidendrum schbomburgkii） were used as materials, and the media formulas which were suitable for the induction, proliferation, germination of the protocorn and root growth were screened. The results revealed that after the seeds of tree orchid was sown on the media MS ＋ BA0.5 mg · L^-1 ＋ NAA0.25 mg· L^-1 ＋ sugar 25 g· L^-1, after 7--10 days, there are some green spots, after 25 days, the protocorn could been seen growing on it; the media 1/2MS ＋6 - BA1.5 mg · L^-1 ＋ NAAO. 25 mg · L^-1 ＋ sugar20 g · L^-1＋ CH2.0 g · L^-1 is most suitable for the proliferation of protocorn, with the best effectiveness and the proliferating coefficient over 9 times; that the germination rate attain over 90% by implanting the protocorn in the media 1/2MS ＋ NAA0.1 mg· L^-1 ＋6 - BA0.2 mg· L^-1 ＋ sugar 25 g · L^-1 ; the rooting percentage of 94% could also be obtained by implanting the germinated buds in the media 1/2MS ＋ NAA 0.5 mg · L^-1 ＋ BA 0.1 mg· L^-1＋banana 70 g · L^-1 ＋sugar 25 g· L^-1 for rooting; during the period of transplantation, the moss is a satisfied medium for transplanting by which the survival rate can reach as high as 92.5%, and the planflets vigorously grow.