女贞ISSR分子标记的引物筛选

Primer Screening in the Study on Ligustrum lucidum Ait. with ISSR Markers

利用60个ISSR引物,对来自不同地域的12份女贞种质材料进行PCR扩增,筛选出12个多态性丰富、条带清晰且重复性良好的、适合于所有女贞种质材料进行ISSR分析的有效引物。筛选出的12个有效引物对12份女贞种质材料进行ISSR-PCR扩增,均可获得带型丰富和清晰可辨的DNA指纹图谱,共扩增出228条DNA谱带,其中210条为多态性带,占总扩增带数的92.1%,平均每个引物扩增出19条谱带。本试验筛选的12条引物可以有效地应用于女贞种质资源材料的ISSR分析。

50 ISSR primers were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait. from different areas, and 12 effective ISSR primers which were suitable for all gerplasm materials of Ligustrum lucidum Ait. were selected out, which were used in the ISSR-PCR amplification for 12 germplasm materials of Ligustrum lucidum Ait. The results indicated that 228 distinct DNA bands are obtained, and 210 bands are polymorphic, which amount to 92.1% of the total bands amplified. Averagely, 19 bands could be obtained with each primer. The 12 primers selected could be effectively applied to ISSR analysis of the germplasm materials of Ligustrum lucidum Ait.