摘要
目的探讨改良荧光原位杂交(fluorescent in situ hybridization,FISH)技术在检测乳腺癌HER2基因中的应用。方法对52例经免疫组化(immunohistochemistry,IHC)法检测HER2的乳腺癌标本行改良FISH法检测HER2基因扩增情况,Kappa检验法对两种检测结果的一致性进行统计分析。结果改良FISH法成功率高,与IHC法检测结果一致性好,两者实际一致率为88.5%(K=0.757,P=0.000)。结论改良FISH技术可作为最终确诊HER2基因状态的方法 。
Objective To investigate the implication of improved fluorescent in situ hybridization (FISH) technique in the detection of HER2 gene in breast cancer tissues.Methods HER2 genes amplification in 52 samples with breast cancer detected by IHC method was tested by improved FISH method.Kappa test was employed to compare the results of the two methods.Results Improved FISH method had high success rate and high coincidence with IHC method.The concordance rate was 88.5% (K=0.757,P=0.000).Conclusion The improved FISH is simple to operate,safe,stable,and highly sensitive in detecting HER2 gene amplification of breast cancer tissue.It can be utilized to diagnose HER2 gene status.
出处
《华南国防医学杂志》
CAS
2010年第3期208-210,共3页
Military Medical Journal of South China
关键词
荧光原位杂交
免疫组化
乳腺癌
HER2基因
Fluorescence in situ hybridization
Immunohistochemistry
Breast cancer
HER2 gene