RNA沉默Slug基因对人乳腺癌细胞株MCF-7侵袭及血管生成的影响

Effect of RNA silent Slug gene on invasion and angiogenesis of human breast cancer cell line MCF-7

目的:研究Slug-shRNA-1干扰Slug基因对MCF-7侵袭潜力及诱导血管内皮细胞形成管腔能力的影响。方法:通过体外侵袭模型,测定MCF-7通过Slug-shRNA-1作用于Slug基因后穿透Matrigel的潜力;应用MCF-7与人脐静脉内皮细胞(HUVEC)双室联合培养技术,观察MCF-7通过Slug-shRNA-1作用于Slug基因后诱导HUVEC形成管腔能力的影响。结果:siRNA-Slug作用于Slug基因后能明显降低MCF-7穿透Matrigel的能力(P<0.05),抑制MCF-7诱导HUVEC形成管腔样结构的能力(P<0.05)。结论:Slug-shRNA-1作用于Slug基因后能明显降低MCF-7的侵袭潜力,抑制MCF-7诱导管腔形成能力。

Objective： To study the effect of Slug-shRNA-1 interference Slug gene on invasive potential and the ability of indu- cing vascular endothelial cells to form vessel lumen. Methods： The potential of MCF - 7 penetrating matrigel after acting on Slug gene by Slug - shRNA - 1 according to in vitro invasion model; the ability of MCF - 7 inducing human umbilical vein endothelial cells to form vessel lumen was observed by dual - chamber culture of MCF - 7 and human umbilical vein endothelial cells. Results： siRNA - Slug reduced the ability of MCF - 7 penetrating matrigel after acting on Slug gene （ P 0. 05） ,suppressed the ability of MCF - 7 inducing human umbilical vein endothelial cells to form vessel lumen （ P 0. 05） . Conclusion： Slug - shRNA - 1 can reduce the invasion of MCF - 7 after acting on Slug gene and suppress the ability of MCF - 7 inducing formation of vessel lumen.