主动免疫治疗对反复自然流产患者外周血Th1/Th2模式表达影响

Effect of active immune therapy on expression of Th1 /Th2 mode in peripheral blood of patients with recurrent spontaneous abortion

目的:探讨主动免疫治疗对原因不明性反复自然流产(URSA)患者Th1/Th2模式表达的影响。方法:采用流式细胞仪对30例URSA患者进行主动免疫治疗前后外周血Th1/Th2模式测定,以30例正常已生育妇女作为对照。结果:UR-SA组Th1型细胞因子IFN-γ(18.98±5.37)%,明显高于对照组的(8.69±2.66)%(P<0.05);而Th2型细胞因子IL-4(5.52±0.18)%,明显少于对照组的(6.78±0.61)%(P<0.05),Th1/Th2为3.14,模式向Th1偏移。主动免疫治疗后Th2型细胞因子IL-4水平(10.27±2.04)%及Th1型细胞因子IFN-γ水平(11.73±2.47)%,Th1/Th2为1.41,模式向Th2偏移。结论:URSA患者Th1/Th2模式偏向Th1,主动免疫治疗可使Th1/Th2模式偏向Th2,诱导母胎免疫耐受,使妊娠成功。

Objective: To explore the effect of active immune therapy on expression of Th1/Th2 mode in peripheral blood of patients with unexplained recurrent spontaneous abortion ( URSA) . Methods: Th1 /Th2 modes in peripheral blood in 30 patients with URSA before and after active immune therapy were detected by flow cytometry,30 normal fertilized women were selected as control group. Results: The level of interferon - γ ( IFN - γ) in Th1 cells of URSA group was ( 18. 98 ± 5. 37) % ,which was significantly higher than that of control group ( 8. 69 ± 2. 66) % ( P < 0. 05) ; the level of interleukin - 4 ( IL - 4) in Th2 cells of URSA group was ( 5. 52 ± 0. 18) % ,which was significantly lower than that of control group ( 6. 78 ± 0. 61) % ( P < 0. 05) ; the ratio of Th1 /Th2 was 3. 14,the mode migrated to Th1. After active immune therapy,the level of IL - 4 in Th2 cells was ( 10. 27 ± 2. 04) % ,and the level of IFN - γ in Th1 cells was ( 11. 73 ± 2. 47) % ; the ratio of Th1 /Th2 was 1. 41,the mode migrated to Th2. Conclusion: Th1 /Th2 mode of patients with URSA migrates to Th1, while Th1 /Th2 mode migrates to Th2 after active immune therapy,then immune tolerance of mothers and fetuses is induced,and success pregnancy is got.