蛇床子素对人乳腺癌细胞增殖、细胞周期及凋亡的影响(英文)

Effects of Osthole on the Proliferation,Cell Cycle and Apoptosis in Human Breast Cancer Cells

蛇床子素是从传统的中药蛇床子的果实中提取的香豆素类衍生物.在发达国家以及发展中国家,乳腺癌是发病率和致死率较高的女性肿瘤之一.本研究的目的是调查蛇床子素对人乳腺癌细胞的增殖、细胞周期以及凋亡的影响.蛇床子素的抗细胞增殖活性用MTT法测定.细胞周期的分布以及细胞凋亡用流式细胞术测定.本研究结果表明,蛇床子素对抑制乳腺癌细胞的增殖、促进G1期阻滞以及诱导细胞凋亡有明显作用.该结果提示有必要进一步研究和评估蛇床子素在乳腺癌治疗中的作用.

Osthole,a natural coumarin derivative,is extracted from the fruit of Cnidium monnieri（L.） Cusson,a therapeutic agent in traditional Chinese medicine.Breast cancer is one of the most commonly diagnosed cancers and the leading cause of death in women,in developed and increasingly in developing countries.The objective of this study is to investigate the effects of Osthole on the proliferation,cell cycle and apoptosis in human breast cancer cells.Antiproliferative activity of Osthole was measured with the 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay.Flow cytometry was performed to observe cell cycle distribution and apoptotic body appearance.Our results show that Osthole is effective in inhibiting cell proliferation,promoting G1 phase arrest and inducing apoptosis in breast cancer cells.These findings indicate a need for further evaluating Osthole as an antitumor agent to treat breast cancer patients.