假丝酵母性阴道炎的病原菌检测及RAPD分析

Pathogen detection and RAPD analysis of candidal vaginitis

目的:探讨女性假丝酵母性阴道炎的病原菌种类,分析其随机扩增DNA多态性(RAPD),为该病的临床诊断及流行病学监测提供依据。方法:刮取106例疑似假丝酵母性阴道炎患者阴道分泌物,分离培养并鉴定病原菌;利用10种随机引物(RAPD1～10)对分离病原菌进行RAPD分析。结果:①分离获得假丝酵母72株,其中白假丝酵母56株(77.78%),非白假丝酵母16株(22.22%),包括光滑假丝酵母6株(8.33%)、热带假丝酵母4株(5.56%)、克柔假丝酵母1株(1.39%)及其他假丝酵母5株(6.94%);②RAPD分析表明:有2种引物(RAPD2和RAPD5)可以获得较清晰、稳定的特异性带型,具有较明显的种间遗传变异性和种内遗传相似性。结论:假丝酵母性阴道炎病原菌以白假丝酵母为主。随机引物RAPD2和RAPD5比较适于白假丝酵母和热带假丝酵母的分型、鉴定。

Objective To approach the spectrum of pathogen of candidal vaginitis and analyze the randomly amplified polymorphism DNA （RAPD） of isolated pathogen,and provide basis for clinical diagnosis and epidemiological surveillance of candidal vaginitis.Methods The specimens of vaginal discharge of 106 patients suspected with candidal vaginitis were collected.Pathogenic fungal were isolated and identified.10 random primers （RAPD 1-10） were used to make RAPD analysis on the isolates.Results ①72 strains of Candida were isolated,among which C.albicans was 56 strains （77.78%）,non-Candida albicans Candida （NCAC） species was 16 strains （22.22%） including 6 strains of C. glabrata （8.33%）,4 strains of C. tropicalis （5.56%）,1 strains of C. krusei （1.39%） and 5 strains of other Candida sp. （6.94%）.②RAPD analysis result showed that 2 primers （RAPD2 and RAPD5） could have better clear and stabile specific pattern bandings with evident interspecies genetic variability and intraspecies genetic resemblance.Conclusion C.albicans is the main pathogen of candidal vaginitis.RAPD2 and RAPD5 are suitable for identification and typing of C.albicans and C.tropicalis.