STAT3基因在人肝癌细胞株HepG2、Bel7402、SMMC7721和肝癌组织中的表达及意义

Expressions of STAT3 gene in human hepatocellular carcinoma cell lines HepG2,Bel7402,SMMC7721 and hepatocellular carcinoma tissues and their significances

目的:探讨STAT3基因异常活化在人原发性肝癌发生、发展中的作用及可能的机制。方法:应用免疫组化染色法、RT-PCR和Western blotting法检测人肝癌细胞株HepG2、Bel7402、SMMC7721和肝癌组织中STAT3基因的mRNA和蛋白水平表达。结果:肝癌细胞株SMMC7721、Bel7402和HepG2中均有STAT3基因的高表达,3种肝癌细胞间表达量比较差异无显著性(P>0.05)。原发性肝癌组织和癌旁组织中均有STAT3的mRNA及蛋白水平的高表达,与正常肝组织比较差异有显著性(P<0.05),而肝癌组织与癌旁组织比较差异无显著性(P>0.05)。肝癌组织中VEGF、survivin和c-myc的mRNA表达上调,p53的mRNA表达下调。结论:STAT3的异常活化可能发生在癌变的早期,在肝癌的形成和发展中可能起重要的促进作用。

Objective To explore the role and possible mechanism of abnormal activation of STAT3 gene during the occurrence and development of human hepatocellular carcinomar（HCC）.Methods Immunohistochemistry dyeing,RT-PCR and Western blotting were used to detect the mRNA and protein expressions of STAT3 gene in HCC cell lines SMMC7721,Bel7402 and HepG2 and human HCC tissues.Results There were high expressions of STAT3 gene in SMMC7721,Bel7402 and HepG2 cells,and there was no significant difference among them（P0.05）.There were high expressions of STAT3 gene at the mRNA and protein levels in HCC tissues and tissues surrounding carcinoma,and there was significant difference between normal tissues and HCC tissues（P0.05）,while there was no difference between HCC tissues and tissues surrounding carcinoma（P0.05）.The mRNA and protein expressions of VEGF,survivin and c-myc genes were increased in HCC tissues,while the mRNA and protein expressions of p53 were decreased in HCC tissues.Conclusion The persistent activation of STAT3 gene may occur at early stage of HCC pathogenesis and plays an important promoting role in the carcinogenesis of HCC.