卵巢浆液性囊腺癌组织中β-连环蛋白的表达及其意义

Expression of β-catenin in ovarian serous cystadenocarcinoma tissues and its significances

目的:研究卵巢浆液性囊腺癌组织中β-连环蛋白(β-catenin)的表达,阐明β-连环蛋白在卵巢浆液性囊腺癌诊断中的临床意义。方法:采用RT-PCR法检测36例卵巢浆液性囊腺癌、10例卵巢浆液性囊腺瘤及10例正常卵巢组织β-连环蛋白mRNA的表达量。应用免疫组织化学方法检测β-连环蛋白在60例卵巢浆液性囊腺癌、24例卵巢浆液性囊腺瘤及24例正常卵巢组织中的表达率。结果:β-连环蛋白mRNA在卵巢浆液性囊腺癌组表达量为1.001±0.192,在正常卵巢组为0.588±0.128,在卵巢浆液性囊腺瘤组为0.600±0.262,卵巢浆液性囊腺癌组β-连环蛋白mRNA的表达量显著高于正常卵巢组和卵巢浆液性囊腺瘤组,差异有显著性(P<0.05)。β-连环蛋白主要在细胞质中表达,在卵巢浆液性囊腺癌组的β-连环蛋白阳性表达率为88.33%,明显高于正常卵巢组的20.83%和卵巢浆液性囊腺瘤组的33.33%(P<0.05);在卵巢癌组临床分期Ⅰ的卵巢癌β-连环蛋白阳性表达率为77.77%,临床分期Ⅳ期的卵巢癌β-连环蛋白阳性表达率为93.87%,两者比较差异有显著性(P<0.05);在卵巢癌病理分级G1、G2及G3组的β-连环蛋白阳性表达率分别为85.00%、89.28%和91.63%,组间比较差异无显著性(P>0.05)。结论:卵巢癌组织中β-连环蛋白mRNA和蛋白表达均增高,β-连环蛋白表达程度与卵巢癌临床分期相关,与病理分级无关,β-连环蛋白在卵巢癌诊断中起重要作用。

Objective To study the expression of β-catenin in ovarian serous cystadenocarcinoma tissues and disscuss the clinical significances of β-catenin in the diagnosis of ovarian serous cystadenocarcinoma.Methods RT-PCR method was used to detect the expressions of β-catenin mRNA in tissue samples of 36 ovarian serous cystadenocarcinoma,10 ovarian serous cystadenoma and 10 normal ovary tissues.The expressions of β-catenin in 60 ovarian serous cystadenocarcinoma,24 ovarian serous cystadenoma and 24 normol ovarian tissues were detected by immunohistochemistry.Results The expression level of β-catenin mRNA in ovarian serous cystadenocarcinoma tissues（1.001±0.192）was significantly higher than those in normal ovarian tissues（0.588±0.128）and ovarian serous cystadenoma tissues（0.600±0.262）（P0.05）.The expression of β-catenin was mainly located in cytoplasm;the positive rate of β-catenin in ovarian serous cystadenocarcinoma group was 88.33%,which was significantly higher than those in normal ovarian group（20.83%）and ovarian serous cystadenoma group（33.33%） （P0.05）.There was significant difference in the expression level of β-catenin in ovarian cancer between different clinical stages,while the positive rate in stage Ⅰ was 77.77% and in stage Ⅳ was 93.87%（P0.05）;however,there was no significant difference in the expression level of β-catenin in ovarian cancer between different pathlogical grades,while the positive rates in ovarian cancer for G1,G2 and G3 were 85.00%,89.28%,and 91.63%,respectively;there was no significant difference between various groups（P0.05）.Conclusion The expression level of β-catenin in ovarian cancer is higher,the expression level of β-catenin is associated with the clinical staging of ovarian cancer but unrelated with pathological grade;β-catenin may play an important role in the detection and diagnosis of ovarian cancer.