载脂蛋白E及胆固醇酯转运蛋白基因多态性与冠心病的相关研究

Interaction between ApoE and cholesteryl ester transfer protein polymorphism and effects on coronary heart disease

目的探讨载脂蛋白E(ApoE)基因多态性和胆固醇酯转运蛋白(CETP)基因多态性与冠心病发生风险的关系以及二者有无交互作用。方法筛选冠心病患者121例及非冠心病者107例为研究对象,应用聚合酶链反应限制片长多态性分析方法检测ApoE基因多态性和CETP基因多态性。结果冠心病组ApoE4等位基因频率(10.7%)显著高于对照组(4.7%,P<0.05);CETP基因型频率在两组间差异无统计学意义(P>0.05)。ApoE4是冠心病的独立危险因素(OR=2.502,95%CI1.017-6.156,P=0.046)。CETP基因型及其与ApoE的交互作用与冠心病发生风险无显著性关系(P>0.05)。结论ApoE4基因型与高TC、LDL-C水平有密切关系。冠心病患者ApoE4等位基因频率显著高于对照组。等位基因E4是冠心病的独立危险因素。CETP基因型频率在冠心病组和对照组无显著性差异。CETP基因型及其与ApoE基因型的交互作用对冠心病的发生无显著性影响。

Objective To determine the frequency of the ApoE and cholesteryl ester transfer protein（CETP） polymorphism in Chinese,and to investigate its relationship with plasma lipid levels and coronary heart disease（CHD）.Methods In this case-control study,ApoE and CETP-TaqIB polymorphism were examined in 121 patients with CHD and 107 non-CHD controls.Polymerase chain reaction-restricted fragments length polymorphism was used to determine ApoE and CETP genotype.Results ApoE4 allele was significantly more prevalent in CHD patients（10.7%） than in controls（4.7%,P0.05）.ApoE4 allele was an independent risk factor for CHD（OR=2.502,95%CI 1.017-6.156,P=0.046）.The interaction between ApoE and CETP genotype had no significant effect on CHD.Conclusion It is the first study to explore the effect of interaction between ApoE and CETP genotype and its effect on CHD.ApoE4 allele is an independent risk factor for CHD.The interaction between ApoE and CETP genotype has no significant effect on CHD.