结直肠癌组织BCL9和VEGF的表达与临床意义

The Expression of BCL9 and VEGF in Colorectal Cancer and Their Clinical Significance

探讨结直肠癌中B细胞淋巴瘤因子9(B-cell lymphoma 9,BCL9)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达与临床病理的关系及其临床意义.采用免疫组织化学SABC染色法,检测83例结直肠癌组织和10例癌旁正常组织中BCL9及VEGF蛋白的表达情况,并且分析其与临床病理特征的关系.BCL9和VEGF在癌组织中表达率分别为67.47%(56/83)和69.88%(58/83),而癌旁正常组织中均不表达.BCL9在结直肠癌中的表达与肿瘤分化程度、浸润深度、Dukes分期、有无淋巴结转移相关(P<0.05).VEGF在结直肠癌中的表达与肿瘤分化程度、浸润深度无关,而与肿瘤Dukes分期、有无淋巴结转移密切相关(P<0.01).结直肠癌中VEGF的表达随BCL9表达的升高而升高(P<0.01).BCL9、VEGF可能在结肠癌的发生发展过程中起重要作用.BCL9可能通过上调VEGF的表达,促进肿瘤浸润和转移.

To explore the relationship between the expression of B-cell lymphoma 9（BCL9）and vascular endothelial growth factor（VEGF）in colorectal cancer and their clinical pathological features,and their clinical significance.Immunohistochemistry SABC method was used to detect the expression of BCL9 and VEGF in 83 cases of colorectal carcinomas and 10 cases of cancer adjacent normal tissue,and the relationship between protein expression and clinical pathological features was analyzed.The expression rate of BCL9 and VEGF protein in cancer tissue were 67.47%（56/83）and 69.88%（58/83）,respectively.While both had no expression in cancer adjacent normal tissue.The BCL9 expression was correlated with differentiation degree,depth of infiltration,Dukes stage and lymph node metastasis（P 0.05）.There was no correlation between the VEGF expression and differentiation degree,depth of infiltration,but that was closely correlated with Dukes stage and lymph node metastasis（P0.01）.The expression of VEGF increased with BCL9（P0.01）.BCL9 and VEGF may play an important role in development of colorectal carcinoma.The tumor invasion and metastasis may be promoted by BCL9 which works by up-regulating the expression of VEGF.