麝香滴眼液对实验变应性结膜炎的治疗研究

Treatment of experimental allergic conjunctivitis with musk eye drops in guinea pigs

目的:观察麝香滴眼液对豚鼠变应性结膜炎模型的治疗作用,并探讨其机制。方法:建立由卵清蛋白和氢氧化铝混合液所致的豚鼠变应性结膜炎模型,将造模成功的豚鼠随机分为模型组,麝香滴眼液大、中、小剂量组、市售地塞米松组、和溶媒对照组,共计6组,每组10只。各组用相应药物治疗一周,观察各组豚鼠局部刺激症状,眼组织中组胺和OVA特异性IgE含量以及结膜组织的形态变化。结果:模型组眼部症状评分、搔抓反应计数、眼组织中组胺和OVA特异性IgE含量以及结膜组织形态学变化均较模型组优,麝香大、中、小剂量组明显改善眼局部症状(P<0.05),减少搔抓反应次数(P<0.05);降低眼组织中组胺含量(P<0.01)和OVA特异性IgE含量(P<0.05),减轻结膜组织病理学改变。结论:麝香滴眼液对变应性结膜炎具有良好的治疗作用,其作用机制可能是通过减少特异性IgE的产生而发挥作用。

AIM： To investigate the therapeutic effect and its mechanisms of musk eye drops on allergic conjunctivitis （AC） in guinea pigs.METHODS： AC was developed by intraperitoneal injection of a mixture containing Ovalbumin （OVA） and Al（OH）3 in guinea pig.Sixty guinea pigs with AC were randomly divided into 6 groups,i.e.,AC model group,three treated groups treated with musk eye drops in high,middle and low dosage,group treated with dexamethasone sodium phosphate eye drops.Together with solvent group as sixth group.After one week of administration,the changes of guinea pigs in symptoms,histamine and OVA-specific IgE concentration of ocular tissues,and morphological features of conjunctiva were observed under light microscopy.RESULTS： Compared with the vehicle group,the conjunctiva symptoms scale,histamine and OVA-specific IgE concentration of ocular tissues and morphological changes scale of conjunctiva of guinea pigs in the model group were fairly good.Compared with the model group,the ocular symptoms of guinea pigs in each of the musk eye drops treated groups showed relieved （P 0.05）,histamine concentration of ocular tissues in the treated group released （P 0.01）,and OVA-specific IgE also decreased （P 0.05）,morphological changes of conjunctiva tissues were relieved in the treated groups.CONCLUSION： Musk eye drops is effective in treating AC,and its action mechanism might be related to the decrease in the OVA-specific IgE.