CGC法同时测定两种莪术油在大鼠血浆中莪术醇和莪术二酮的含量

Analysis of curcumol and curdione in plasma of rats by CGC

目的:研究比较莪术油、莪术残油(莪术油提取出β-榄香烯的残余物)在大鼠血浆中莪术醇和莪术二酮的含量。方法:采取大鼠灌胃给药,以对羟基苯甲酸乙酯为内标物,采用毛细管气相色谱法同时测定,内标法计算莪术油、莪术残油中标志性成分莪术醇和莪术二酮的血浆药物浓度,运用DAS2.0分析计算药代动力学参数。结果:莪术醇和莪术二酮分别在0.001028～0.6580mg/mL和0.04064～1.300mg/mL范围内呈现良好的线性关系,精密度、稳定性及融冻次数考察均符合有关要求,测定方法的绝对回收率莪术醇为72.3%～85.3%,莪术二酮为70.1%～87.0%;内标物对羟基苯甲酸乙酯的绝对回收率为74.9%～96.5%;方法回收率莪术醇为55.8%～69.0%,莪术二酮为61.6%～68.3%,均符合有关要求。通过药时曲线和药代动力学参数分析,莪术油、莪术残油中莪术醇、莪术二酮在大鼠的体内代谢过程基本符合二房室模型。结论:在莪术油和莪术残油中莪术醇的血药浓度变化相似,而莪术二酮血药浓度存在较大差异。

AIM： To compare the content difference in rat＇s plasma concentration of curcumol and curdione between zedoary oil and its residue oil（the latter come from residum after removing β-elemene.METHODS： After the zedoary oil and its residue oil was administered,ethyl-p-hydroxybenzoate in rat＇s plasma used as internal standard was carried out simultaneously to determine curcumol and curdione by capillary column gas chronatography.RESULTS： A good linearity was obtained when the concentration of Curcumol ranged from 0.001 028 to 0.658 0 mg/mL,and the concentration of Curdione ranged from 0.040 64 mg/mL to 1.300 mg/mL.Precision,stability and the study of the number of thawing and freezing test were within the requirements.The recovery of curcumol was 72.3%-85.3% ,the recovery of Curdione was 70.1%-87% .The recovery of internal indicator was in the range of 74.9%-96.5% .The Curcumol’s methods recovery was in the range of 55.8%-69% ,the Curdione’s methods recovery was in the range of 61.6%-68.3% .Recovery experiments were within the requirement.Two-compartment model of curcumol and curdione was fit to metabolic processes in vivo in rats drug concentration-time curve and pharmacokinetic parameters.CONCLUSION： Curcumol’s changes of both zedoary oil and residue oil in plasma concentration are similar in blood concentration,but curdione’s changes shows great difference.Two indicators make difference in pharmacokinetic parameters.