麦胚蛋白酶解物清除自由基及抗氧化作用的研究

Elimination Effect on the Free Radical and Antioxidation Effect of Wheat Germ Protein Hydrolysates

研究麦胚蛋白酶解物的体外抗氧化活性。采用邻苯三酚自氧化、Fenton反应及FTC法分析酶解物清除自由基及抗脂质过氧化的效果。结果表明:麦胚蛋白酶解产物对三种自由基均有一定的清除活性,量效关系明显;其清除O2-·、·OH、DPPH·三种自由基的IC50分别为6.2、3.5、2.3mg/mL;在亚油酸体系中具有良好的抗氧化效果。麦胚蛋白酶解物是有效的、多功能的天然抗氧化剂和自由基清除剂,具有综合开发利用的价值。

Researched the antioxidantion activeness in vitro of wheat germ protein enzymolysis. Analysis the effect of enzymolysis scavenging radical and the anti-lipin peroxidation by using the pyrogallol system, the Fenton reaction and the FTC. The result indicated that： The Enzymolysis product of wheat germ protein has certain scavenging activeness to three kind of radicals, and has obvious effect-relations. It scavenge the IC50 of O2·,·OH, DPPH.three kind of radical is： 6.2, 3.5, 2.3 mg/mL, respectively; It has the well antioxidantion effect in the linoleic acid system. The enzymolysis of wheat germ protein is effective, the multi-purpose natural antioxidantion and the radical scavenger, has the comprehensive development using value.