酶解法制备鱼籽多肽及清除自由基的研究

Study on Preparation and Free Radical-scavenging Activity of Hydrolysate of Fish Seeds

鱼籽含有丰富的蛋白质,具有较高的开发价值。该文对海洋鱼的鱼籽进行脱脂酶解,考察酶解液清除自由基的能力。蛋白酶筛选结果表明,木瓜蛋白酶酶解产物对羟自由基的清除率最高可达到77.3%。底物浓度1:2时的清除率为88%,加酶量300U/g时的清除率为89%,50℃时的清除率为90.5%,酶解时间40min时的清除率为85%,pH6.0时的清除率为98.5%。通过正交试验优化得到清除自由基肽的酶解工艺条件为:用木瓜蛋白酶在温度50℃、酶解时间40min、pH6.0、加酶量300U/g、料水比1:2的条件下,鱼籽酶解液的羟自由基清除率最大,为94.2%。层析分离的结果有2个明显的洗脱峰。

fish seeds which are rich in protein has a high development value. The article study on the components of the hydrolysates from marine fish seeds which lipid is removed, the hydrolysis of small peptides have a certain anti-oxidation which free radicals is removed by small peptides. This paper is the determination of the hydroxyl radical clearance rate. Select the three enzymes which is papain, wgioipojg and trypsin to hydrolysis. The results showed that clearance rate of the hydroxyl radical of.papain solution is up to 77.3 %. Further the effects of substrate concentration, volumes of enzyme, temperatures and times on the preparation of removing free radicals peptides is studied. The best single factors measured scavenging rates are as follows： The clearance rate of 1：2 substrate concentration is 88%, 300 U/g enzyme volume 89%, hydrolysis temperature 50℃ 90.5%, hydrolysis time 40min 85 %, hydrolysis pH6.0 98.5%. The enzymic processesing conditions through the by orthogonal test optimization are as follows： hydrolysis time 40min, hydrolysis temperature 50℃, hydrolysis pH6.0, the clearance rate was 94.2% in optimization conditions. The result of fractionation had two fraction bands.