反相高效液相色谱法分离制备银杏内酯

Separation and Preparation of Ginkgolides by Reverse Phase-highperformance Liquid Chromatography

采用高效液相色谱技术,分离制备高纯度银杏内酯。通过单因素及正交试验,对银杏内酯的浸提条件进行优化,再通过反相高效液相色谱分离系统,采用浓度梯度洗脱的方式,分离出高纯度银杏内酯。结果显示最佳浸提条件:提取剂为乙酸乙酯,浸提时间4h,温度65℃,固液比1:5,银杏内酯含量由原料中的6%,提高到29%。通过制备色谱柱层析,得到产品中银杏内酯纯度≥90%,回收率为70%。

Separation and preparation of high purity of ginkgolides from Ginkgo biloba leaves was investigated by high-performance liquid chromatography （HPLC）. The efficient factors of extraction of ginkgolides by single factor tests and orthogonal experiment were optimized. RP-HPLC using gradient elution method was utilized. And then the high purity ginkgolides was achieved from the crude extract of Ginkgolide. Results showed： the optimum conditions of extraction as follows： the extractant of ethyl acetate, extraction temperature 65~（3, extraction time 4 h, ratio of solid to liquid extraction 1：5. The content of ginkgolides was increased from 6% to 29%. The product of ginkgolides was obtained by RP-HPLC, product purity was about 90%, product yield was 70%.