摘要
研究了DTPA-BIS-BIOTIN的177Lu标记方法,优化了标记条件,并进行了标记物在正常小鼠体内分布实验。在最佳标记条件下(DTPA-BIS-BIOTIN 25μg,标记介质pH=4.5,80℃反应20 min),177Lu-DTPA-BIS-BIOTIN标记率大于99.0%,室温下放置96 h,标记物体外稳定性良好。正常小鼠体内分布实验结果表明,177Lu-DTPA-BIS-BIOTIN在血液中清除快,主要浓集于肝、脾和肾,经肾脏排泄。本研究为进一步采用177Lu-DTPA-BIS-BIOTIN进行肿瘤预定位显像及治疗研究提供了实验基础。
The labeling method for 177Lu-DTPA-BIS-BIOTIN was established,and the biodistribution of 177Lu-DTPA-BIS-BIOTIN in normal mice was carried out as well.Under the optimal experimental condition（DTPA-BIS-BIOTIN 25 μg,pH=4.5 reacting at 80 ℃ for 20 min）,the labeling yield of 177Lu-DTPA-BIS-BIOTIN is more than 99.0%.177Lu-DTPA-BIS-BIOTIN shows pretty good in vitro stability.The biodistribution of 177Lu-DTPA-BIS-BIOTIN in normal mice shows a rapid blood clearance.The uptake of 177Lu-DTPA-BIS-BIOTIN is mainly accumulated in liver,spleen and kidney.177Lu-DTPA-BIS-BIOTIN is excreted by kidney.The results provide the basis for further study on 177Lu-DTPA-BIS-BIOTIN used in pretargeted radioimage and radiotherapy of cancer.
出处
《核化学与放射化学》
CAS
CSCD
北大核心
2010年第3期184-188,共5页
Journal of Nuclear and Radiochemistry
基金
国家自然科学基金资助项目(30670584)
