来航鸡Mx基因14个外显子克隆及测序

Cloning and Sequencing 14 Exons of Mx Gene from White Leghorn

禽类的Mx蛋白具有抵抗禽流感病毒及水疱性口炎病毒(VSV)的功能,对禽类Mx蛋白基因进行研究具有重要意义。以白来航(White Loghorn,WLH)作为实验动物,获得Mx蛋白基因全长并对基因组结构进行分析并克隆测序获得了Mx基因所有外显子区(1-14exons),为研究Mx基因结构及多态性分析奠定了基础。

Avian Mx protein was discovered to have antiviral activity（avian influenza and vesicular stomatitis）.Because birds are natural hosts of avian influenza,the study of chicken Mx protein and its encoding genes is of significance.In the present study,White leghorns（WLH） were selected to obtain the full length of Mx cDNA and the genome structure was also analysised.All of the 14 exons were obtained and senquenced.The research forms the basis for the genome structure and polymorphism of Mx gene.