摘要
在实验室建立了分散的新生大鼠海马神经细胞体外培养模型。该模型属原代培养，在体外能直接观察神经元的生长发育。研究表明：该模型稳定性好，差异性小；培养神经细胞可存活１个月以上，１４ｄ左右细胞生长最为丰满，且四周晕光明显，神经突起多而粗大，分枝互成网络；１４ｄ以后生长减缓。由于在此模型培养的条件下，既不存在血脑屏障问题，又容易控制条件，便于在细胞和分子水平上深入研究神经细胞的形态学、生理学、生化代谢及药理学等方面的问题，特别适合在体外进行神经药理学的研究。
The model cultured newborn rat hippocampal neuron was established. This model is a primary culture which preserves the structure characteristic and biological activities of neuron in vivo. Extensive processus elongation and arborization form a network after several days in culture. There is no blood brain barrier in this model. It is conceivable to observe the neuronal growth and development and response to external stimuli in morphology, physiology, biological metabolism, and pharmacology.
