He-Ne激光对增强UV-B辐射后小麦叶片蛋白及核酸影响的研究

Influence on Soluble Protein and Nucleic Acid of Leaves in Wheat Seedings Exposed to Enhanced Ultraviolet-B Radiation and Irradiated by He-Ne Laser

采用He-Ne激光辐照对增强UV-B辐射后小麦幼苗的损伤修复作用进行了研究.小麦种子在盛有湿滤纸的培养皿内25℃下进行萌发.萌发后小麦幼苗在经10.08kJ·m-·2d-1的增强UV-B辐射,然后再用10mW·mm-2的He-Ne激光进行辐照.通过测定小麦幼苗叶片中可溶性蛋白、核酸含量以及相关酶活性的变化,研究了He-Ne激光对小麦UV-B损伤的修复作用.结果显示,可溶性蛋白、核酸含量、硝酸还原酶含量及活性的变化同小麦幼苗损伤修复的能力相关联.增强UV-B辐射抑制小麦生长,降低小麦生物量和小麦叶片可溶性蛋白及核酸含量.小麦叶片蛋白水解酶活性上升、硝酸还原酶活性下降、叶片总游离氨基酸含量增加.通过He-Ne激光辐照,可溶性蛋白及核酸含量提高,蛋白水解酶活性增高程度有所缓解,而硝酸还原酶活性降低的程度有所上升.

This paper studied the effects and mechanism of He-Ne laser （5mW.mm-2）irradiation on the variation of thesoluble Protein and nucleic acid in damaged wheat leaves induced ultraviolet-B （10.08kJ.mm-2.d-1）radiation. It implied that underenhanced ultraviolet-B could decrease the biomass,the contents of soluble Protein and nucleic acid. The activities of proteinaseand the content of total free amino acids increases,while the activity of NRase decreases. While all the damages of wheat seedlingsinduced by enhanced UV -B radiation in physiology could be repaired partly by He -Ne laser irradiation with decreasing theactivities of proteinase and the content of total free amino acids of wheat seedings＇ leaves,increasing the content of soluble Proteinand nucleic acid.. This is the first investigation reporting the use of laser irradiation treatment to repair the wheat damage inducedby UV-B radiation in physiology aspect.