口气清新泡腾片定性定量标准研究

Qualitative and quantitative evaluation standard of Kouqi Qingxin Paotengpian

目的制定口气清新泡腾片的质量标准。方法采用薄层色谱法（TLC）鉴别方中山楂,采用高效液相色谱法（HPLC）测定金银花中绿原酸的含量,100-5-C18色谱柱（250 mm×4.6 mm,5μm）,流动相：0.4%磷酸溶液-乙腈（87∶13）,流速：1 mL.min^-1,检测波长：327 nm,柱温：30℃。结果 TLC法可检出山楂,斑点清晰,阴性样品无干扰,专属性强。绿原酸在0.122 4-0.367 2μg与峰面积线性关系良好（n=5）（r=0.999 8）,平均加样回收率为100.4%,RSD为1.7%。结论该方法简便、可靠、准确,可用于口气清新泡腾片的质量控制。

Objective To improve the quality standard of Kouqi Qingxin Paotengpian.Methods Hawthorn in the tables were identified by thin-layer chromatography,the content of chlorogenic acid was determined by HPLC,the separation was performed on an 100-5-C18（250 mm×4.6 mm,5 μm）,the mobile phase consisted of 0.4% phosphoric acid-acetonitrile（87∶13）,the detective wavelength was 327 nm,the flow rate was 1.0 mL·min^-1,and the column temperature was 30℃.Results Hawthorn could be identified by thin-layer chromatography,and the identification was highly specific without interference.The linear range of chlorogenic acid was 0.122 4-0.367 2 μg.The average recovery was 100.4%,RSD=1.7%.Conclusion The method is simple,reliable,and accurate,and can be used for the quality control of Kouqi Qingxin Paotengpian.