高效液相色谱法测定射干利咽口服液中射干苷、次野鸢尾黄素的含量

Determination of belamcandin and irisflorentin in Shegan Liyan Koufuye by HPLC

目的建立高效液相色谱同时测定射干利咽口服液中射干苷、次野鸢尾黄素含量的方法。方法采用Dia-monsil C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈（A）-0.1%磷酸（B）（pH=2.25）,进行梯度洗脱,0 min：20%（A）;20 min：60%（A）;40 min：20%（A）;流速：1.0 mL·min^-1;进样量：20μL;紫外检测波长：265 nm;柱温：室温。结果射干苷和次野鸢尾黄素保留时间分别为20.5和35.5 min,与各自相邻峰的分离度均〉1.5。以峰面积对进样浓度（ng·mL^-1）线性回归,射干苷回归方程：Y=7 485.5X＋82.95,r=0.999 7,线性范围：150-3 000 ng·mL^-1;次野鸢尾黄素回归方程：Y=2 031X-78.14,r=0.999 9,线性范围：50-1 000 ng·mL^-1。射干苷和次野鸢尾黄素的回收率分别为97.2%和98.7%、RSD分别为2.1%和2.8%。结论本方法操作简便,测定结果准确可靠,可用于射干利咽口服液中射干苷、次野鸢尾黄素的含量测定。

Objective To establish an HPLC method for the determination of belamcandin and irisflorentin in Shegan Liyan Koufuye.Methods The chromatographic conditions included column Diamonsil C18（4.6 mm ×250 mm,5 μm） with acetonitrile（A）-0.1% phosphoric acid（B）（pH=2.25） as the mobile phase gradient elution.0 min： 20%A;20 min： 40%A;40 min： 20%A.The flow rate was 1 mL·min-1,the injection volume was 20 μL,the detection wavelength at 265 nm,and room temperature was used.Results The retention time of belamcandin and irisflorentin was about 20.5 min and 35.5 min respectively.The resolution was more than 1.5.The regress equation for belamcandin was Y=7 485.5 X＋82.95,r=0.999 7,and the linear range was 150-3 000 ng·mL^-1.That for irisflorentin was Y=2 031 X-78.14,r= 0.999 9,and the linear range was 50-1 000 ng·mL^-1.The average recovery of belamcandin and irisflorentin was 97.2% and 98.7%,RSD was 2.1% and 2.8% respectively.Conclusion This method is simple,time-saving and accurate.It can be used for the content determination of belamcandim and irisflorenti in Shegan Liyan Koufuye.