FasL表达量与卵巢上皮性癌细胞顺铂敏感性的相关性研究

Correlation between FasL expression and platinum sensitivity in ovary epidermis cancer cells

目的探讨卵巢上皮性癌患者原发灶癌细胞中FasL表达量与细胞体外对顺铂药物敏感性的相关性。方法收集本院手术切除的85例卵巢上皮性癌细胞组织,采用免疫组化法检测癌组织中的FasL表达量,按照FasL表达量的高低分为阴性组、阳性组以及强阳性组。采用MTT法比较不同组细胞对于顺铂药物的敏感性。结果 85例卵巢癌组织中,FasL表达为强阳性标本11例,占12.9%,阳性标本52例,占61.2%,阴性标本22例,占25.9%。顺铂对FasL表达强阳性的肿瘤组织细胞的IC50值为(15.31±1.17)μmol.L-1,对FasL表达阳性的肿瘤组织细胞的IC50为(23.71±2.36)μmol.L-1,对FasL表达阴性的肿瘤组织细胞的IC50为(28.27±3.31)μmol.L-1,三者比较差异有统计学意义(P<0.05)。结论不同的个体肿瘤组织中FasL表达量有差异,FasL在卵巢上皮性癌细胞中的弱表达是导致卵巢上皮性癌细胞耐药的原因之一。肿瘤化疗过程中及时监测FasL在细胞中的表达水平对于分析药物的疗效以及预测药物耐药现象的发生具有积极的临床意义。

Objective To explore the correlation between FasL expression and platinum sensitivity in ovary epidermis cancer cells.Methods A total of 85 ovary epithelial cancer cell tissues were collected and the expression of FasL was detected.The tissues were divided into 3 groups（a negative group,a positive group,and a strong positive group）.MTT method was used to test the platinum sensitivity of the three groups.Results Eleven tissues（12.9%） had negative expression of FasL,52 tissues（61.2%） had positive expression of FasL,and 22 tissues（25.9%） strong positive expression of FasL.The IC50 of the three groups was（15.31±1.17）,（23.71±2.36）,and（28.27±3.31） μmol·L^-1 respectively.There was statistical difference among the three groups（P〈0.05）.Conclusion FasL expression is individually different.Platinum sensitivity is associated with low FasL expression.Monitoring of FasL expression is helpful for the analysis process of drug efficacy and prediction of drug resistance.