高效液相色谱法测定枳壳饮片中柚皮苷、橙皮苷和新橙皮苷的含量

Determination of Content of Narigin and Hesperidin and Neohesperidin in Fructus Auranti Pieces by HPLC

目的:建立高效液相法测定枳壳饮片中柚皮苷、橙皮苷和新橙皮苷定量分析方法。方法:Ultimate 3000 Cl8色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-水(磷酸调pH 3)(23∶77)。检测波长283 nm,流速1.0 mL.min-1,柱温30℃。结果:柚皮苷、橙皮苷和新橙皮苷的线性范围分别为0.316 4～1.582 4μg(r=0.999 9),0.041 2～0.206 0μg(r=0.999 9),0.188 0～0.940 0μg(r=0.999 9),平均回收率均大于96.11%。结论:该方法专属性强,结果准确可靠,重现性好,可用于枳壳饮片中柚皮苷、橙皮苷和新橙皮苷的含量测定,以便更好的控制枳壳饮片的质量。

Objective：To establish the method of determining the content of narigin and hesperidin and neohesperidin in Fructus Auranti pieces by HPLC.Method：Ultimate 3 000 Cl8 column（4.6 mm × 250 mm,5 μm）,mobile phase was acetonitrile-water（pH was adjusted to 3 by phosphoric acid）（23∶77）.Wavelength was 283 nm,flow rate was 1.0 mL·min^-1,column temperature was 30 ℃.Result：naringin,hesperidin and neohesperidin linear ranges were 0.316 4 ～ 1.582 4 μg（r = 0.999 9）,0.041 2 ～ 0.206 0 μg（r = 0.999 9）,0.188 0 ～ 0.940 0 μg（r = 0.999 9）.The average coefficient of recovery were greater than 96.11%.Conclusion：This method is highly specific,accurate and reliable and has good repeatability.It is suitable for the determination of the content of narigin and hesperidin and neohesperidin in Fructus Auranti pieces.It can be widely used for the quality control of Fructus Auranti pieces.