HPLC测定芩丹颗粒中黄芩苷、栀子苷和丹皮酚

Determination of Baicalin,Jasminoidin and Paeonol in Qindan Particles by HPLC

目的:建立HPLC测定芩丹颗粒中黄芩苷、栀子苷和丹皮酚质量分数的方法。方法:采用Agilent ZORBAX SB-C18(4.6 mm×150 mm,5μm)色谱柱;黄芩苷以甲醇-水-磷酸(47∶53∶0.2)为流动相;检测波长280 nm。栀子苷以乙腈-水(15∶85)为流动相;检测波长238 nm。丹皮酚以甲醇-水(45∶55)为流动相;检测波长274 nm。流速均为1.0 mL.min-1。结果:黄芩苷、栀子苷和丹皮酚分别在0.006～0.096,0.005～0.080 mg.mL-1和0.005～0.050 mg.mL-1线性关系良好。平均回收率分别为黄芩苷98.8%,RSD 0.75%(n=9);栀子苷99.7%,RSD 0.94%(n=9);丹皮酚98.4%,RSD 1.41%(n=9)。结论:本方法操作简单、快速、准确、重复性好。

Objective：To bulid the method for determination of baicalin,jasminoidin and paeonol in Qindan Particles by HPLC.Method：An Aigilent ZORBAX SB-C18 column（4.6 mm × 150 mm,5 μm） was used.The mobile phase of baicalin was CH3OH-H2O-P（47∶ 53∶ 0.2）,the detection wave lenghth was set at 280 nm.And the mobile phase of jasminoidin was acetonitrile-H2O（15 ∶ 85）,the detection wave lenghth was set at 238 nm.For paeonol,the mobile phase was CH3OH-H2O（45∶ 55）,the detection wave lenghth was set at 274 nm.All flow rates were 1.0 mL·min^-1.Result：Under each condition,the calibration curves of baicalin,jasminoidin and paeonol were linear at the ranges of 0.006 ～ 0.096 mg.L-1,0.005 ～ 0.080 mg.L-1 and 0.005 ～ 0.050 mg·mL^-1,respectively.And the each average recoveries of the method were 98.8% for baicalin（RSD 0.75%,n = 9）;99.7% for jasminoidin（RSD 0.94%,n = 9）;98.4% for paeonol（RSD 1.41%,n = 9）.Conclusion：The developed method is simple,accurate and repeatable for determination of baicalin,jasminoidin and paeonol in Qin-dan Particles.