氧化苦参碱对急性心肌梗死诱发实验性大鼠心肌重塑的影响

Protective Effect of Oxymatrine on Cardiac Remodeling After Acute Myocardial Infarction Induced by Ligated Coronary Artery in Rats

目的:研究氧化苦参碱(OMT)对大鼠急性心肌梗死诱发实验性心肌重塑的保护作用。方法:采用冠状动脉结扎术复制大鼠急性心肌梗死模型,随机分为模型组、OMT高剂量组、OMT中剂量组、OMT低剂量组、卡托普利组;假手术组只做冠状动脉穿线而不结扎。模型复制8周后分别测血流动力学指标以观察各组大鼠心功能的变化;计算心脏质量参数、心室重构参数,碱水解法测心肌羟脯氨酸含量以分析各组大鼠心肌重塑程度;并对心脏进行大体解剖学观察。结果:模型组大鼠血流动力学指标结果提示心功能显著恶化,心脏质量参数、心室重构参数、心肌羟脯氨酸含量及心脏大体解剖观察提示心肌重塑程度显著增加。与模型组比较,OMT具有改善心肌重塑大鼠心功能,抑制心肌重塑的作用。结论:OMT对急性心肌梗死诱发实验性心肌重塑具有一定的保护。

Objective：To investigate the protective effects of oxymatrine（OMT） on myocardial remodeling after acute myocardial infarction（AMI） in rats.Method：AMI was reproduced in male SD rats by ligation of the left coronary artery.The survived rats were randomly divided into five groups as following：model group,OMT50,25,12.5 mg.kg-1 OMT,50 mg.kg-1 captopril,and sham group which was treated as the model group except the operation of arteria coranaria ligation.After eight weeks ligation,the cardiac function was assayed by hemodynamics parameters,the degree of myocardial remodeling was analyzed by cardiac weight indexes,ventricles remodeling parameters and the content of hydroxyproline.Result：The cardiac functions of rats in model group were significantly exacerbated.Cardiac weight parameter,entricular remodeling parameters,and contents of hydroxyproline in ventricule were significantly increased that means degree of myocardial remodeling is increased.After continuous administration of OMT for 8 weeks,the cardiac functions of the rats in OMT group were significantly ameliorated,and the degree of myocardial remodeling was significantly alleviated.Conclusion：OMT could ameliorate the experimental myocardial remodeling induced by AMI in rats.