清热解毒方对急性细菌性腹膜炎大鼠血清内毒素及细胞因子的影响

Experimental Study on the Effect of Qingre Jiedu Fang on the Levels of Endotoxin and Cytokines in Rats with Acute Intra-abdominal Infection

目的:观察清热解毒方对腹腔感染大鼠血清内毒素及细胞因子水平的影响。方法:Wistar大鼠随机分为4组:对照组、模型组、清热解毒方组、头孢克肟组,ig1次,给药30 min后大肠杆菌ip造成大鼠急性细菌性腹膜炎模型,于6,12,18,24 h后分别检测各组动物血浆内毒素,肿瘤坏死因子α(TNF-α),IL-6,IL-10水平。结果:造模后模型组大鼠血清内毒素,TNF-α,IL-6,IL-10较对照组明显升高(P<0.01),清热解毒方组与头孢克肟组上述指标较模型组明显降低。结论:清热解毒方能有效降低大肠杆菌腹腔感染大鼠血清内毒素及TNF-α,IL-6,IL-10水平。

Objective：To explore the effects of Qingre Jiedu Fang（QRJD Fang） on the secretion of endotoxin,TNF-α,IL-6 and IL-10 in intra-abdominal infection rats.Method：After giving 30 min of i.g QRJD Fang or cefi xime（ANT）,rat model of acute bacterial peritonitis was set up.Endotoxin,tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6） and interleukin-10（IL-10） in serum were determined respectively in 6h,12h,18h,24h after acute bacterial peritonitis in the four groups（control group,model,QRJD Fang group,ANT group）.Result：Compared with the control group,serum endotoxin,TNF-α,IL-6 and IL-10 in the model were increased obviously（P 0.01）,while the indexes in QRJD Fang group and ANT group were decreased（P 〈0.01 or P〈 0.05）.Conclusion：QRJD Fang could obviously inhibit the secretion of inflammatory cytokints,such as endotoxin,TNF-α,IL-6 and IL-10 in the acute intra-abdominal infection rats.