冷饭团对实验性肝纤维化的防治作用及其机制

Research on the Preventive and Therapeutic Effects of Kadsura coccinea on Experimental Hepatic Fibrosis in Rat and the Related Mechanism

目的:探讨冷饭团对实验性肝纤维化的防治作用及其机制。方法:采用CCl4和高脂低蛋白等复合因素诱导大鼠实验性肝纤维化,以秋水仙碱为对照,观察冷饭团对大鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(Alb)、血清透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、羟脯氨酸(Hyp)的影响;并作肝脏病理切片,光镜下观察组织学改变。结果:冷饭团能显著降低实验性肝纤维化大鼠血清ALT,AST活性,升高血清Alb含量;显著降低血清HA,LN,PCⅢ和肝组织Hyp,MDA含量,提高肝组织SOD活性;并能明显减轻大鼠肝细胞坏死、肝脏脂肪变性和胶原纤维增生程度。结论:冷饭团有较好的抗肝纤维化作用,其机制可能与抗脂质过氧化作用有关。

Objective：To discuss the preventive and therapeutic effects of Kadsura coccinea on experimental hepatic fibrosis in rat and to explore its mechanism.Method：The rat model of hepatic fibrosis was induced by giving CCl4,rich fat and poor protein.Having a controlled trial with colchicines,Kadsura coccinea’s effects on alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,albumin（Alb）,hyaluronate acid（HA）,laminin（LN）,type Ⅲ procollagen（PC Ⅲ） in serum and malondialdehyde（MDA）,dismutase（SOD）,hydroxyproline（Hyp） in liver tissue were determined.The pathological section of the rat’s liver was examined under an optical microscope.Result：Kadsura coccinea significantly decreased the activity of ALT,AST and the level of HA,LN,PCⅢ in rat serum,and the level of Hyp,MDA in liver tissue.It also increased the level of Alb in serum and the activity of SOD in liver tissue.The degree of necrosis of liver cells,liver fat’s degeneration and collagen fiber hyperplasia were alleviated significantly.Conclusion：Kadsura coccinea shows significant preventive and therapeutic effects on experimental hepatic fibrosis.The possible mechanism of its preventive and therapeutic effects may be related to its anti-lipid peroxidation.