丹桔胶囊对血管紧张素Ⅱ损伤血管内皮功能的保护作用

Protective Effects and Mechanism of Danju Capsule on Angiotensin Ⅱ-Induced Dndothelial Dysfunction

目的:观察丹桔胶囊对血管紧张素Ⅱ(AngⅡ)损伤血管内皮功能的保护作用。方法:制备大鼠胸主动脉环,观察丹桔胶囊提取剂对抗AngⅡ引起的胸主动脉环收缩反应;AngⅡ刺激人脐静脉血管内皮细胞(HUVEC)以反转录—聚合酶链反应(RT-PCR)方法检测HUVEC的内皮素(ET-1)mRNA表达水平;蛋白质印迹(Western blot)方法检测HUVEC中细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)和核转录因子κB(nuclear factorκB,NF-κB)的活化情况。结果:AngⅡ引起胸主动脉收缩,丹桔胶囊制剂能显著对抗血管收缩作用,下调AngⅡ刺激所致的HUVEC细胞ET-1 mRNA过度表达,抑制ERK1/2磷酸化,同时对NF-的活化表现出显著的抑制作用。p65位移、IκB-α磷酸化降解,丹桔胶囊上述抑制作用均呈现浓度依赖性。结论:丹桔胶囊抑制AngⅡ对血管内皮功能的损伤,其机制与降低ET-1表达,抑制ERK/NF-κB途径活化有关。

Objective：To observe the protective effect of Danju Capsule on vascular endothelial dysfunction induced by angiotensinⅡ（AngⅡ）.Method：The Ang Ⅱ-induced contraction of rat aortic rings was tested in tissue bath.The expression of endothelin（ET）-1 mRNA was evaluated by RT-PCR in human umbilical vein endothelial cells（HUVEC） stimulated by Ang II.The activation of extracellular signal-regulated kinase（ERK） and nuclear transcription factor（NF-κB） and its inhibitor（IκB-α） were detected by Western blot in HUVEC.Result：The results showed that 1 × 10^-7 mol·L^-1 Ang Ⅱ increased contraction of rat aortic rings,ET-1mRNA expression,ERK1 /2 phosphorylation,NF-κB p65 displacement and IκB-α phosphorylation degradation,which were significantly inhibited by Danju Capsule in a dose-dependent manner.Conclusion：Ang Ⅱ may be involved in the initiation and progression of cardiovascular diseases by injuring vascular endothelium directly and causing endothelial dysfunction.Danju Capsule was shown to protect against Ang Ⅱ induced endothelial dysfunction,suggesting that Danju Capsule may play a role in the prevention and treatment of cardiovascular diseases.