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HBV感染胎盘乳腺癌耐药蛋白的表达及意义 被引量：1

Expression and siginificance of Breast cancer resist protein in placenta of patients with HBV infection
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摘要 目的探讨乳腺癌耐药蛋白在HBV感染胎盘组织中的表达及其意义。方法选取HBV感染足月胎盘20例;选择无HBV感染足月胎盘20例作为正常对照组。实时荧光定量RT-PCR分别检测两组胎盘中ABCG2mRNA的表达。蛋白免疫印迹法分别检测两组胎盘中乳腺癌耐药蛋白的表达。结果各组荧光定量PCR检测ABCG2 mRNA的相对表达量为乙肝组(0.424±0.358),正常组(0.144±0.116),两组相比,差异有显著性(P<0.005)。蛋白免疫印迹法检测乳腺癌耐药蛋白表达量为乙肝组(0.4250±0.3106),正常组(0.1129±0.1045),两组相比,差异有显著性(P<0.001)。结论乳腺癌耐药蛋白在HBV感染胎盘组织中表达的升高,可能与乙型肝炎病毒感染有关。 Objective ： To determine the expression of Breast cancer resistance protein in full - term placentas from uninfected （ n = 20） and HBV infected women （n = 20）. Methods： ABCG2mRNA transcripts were quantified by real -time PCR using relative determinations. Expression of Breast cancer resistance protein were determined by western blotting. Results： Relative quantification showed a significant （ P 〈 0. 005 ） increase in ABCG2mRNA transcription in HBV -infected women compared to non - infected women, respectively. Western blotting showed a significant （ P 〈 0. 001 ） increase in the expression of Breast cancer resistance protein. Conclusion： The increase expression of ABCG2mRNA and Breast cancer resistance protein in placenta maybe related to HBV infection.
作者 梁慧超 王自能
机构地区 广州市妇女儿童医疗中心妇产科 暨南大学第一附属医院妇产科
出处 《中国优生与遗传杂志》 2010年第6期20-22,共3页 Chinese Journal of Birth Health & Heredity
关键词 乙型肝炎 胎盘 乳腺癌耐药蛋白 荧光定量PCR 蛋白免疫印迹 Hepatitis B Placenta Breast cancer resistance protein Real - time PCR western blotting
分类号 R737.9 [医药卫生—肿瘤]
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参考文献9

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同被引文献12

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中国优生与遗传杂志
2010年 第6期

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