P53、P16、PTEN、hMLH1蛋白表达与卵巢子宫内膜异位症癌变的关系

Correlation of the protein expression of P16,P53,PTEN and hMLH1 with malignant transformation of ovarian endometriosis

目的:探讨P53、P16、PTEN和hMLH1蛋白的表达及其在卵巢子宫内膜异位症癌变过程中的变化和意义。方法:用免疫组织化学染色法检测32例卵巢子宫内膜异位症癌变患者(癌变组)、17例卵巢子宫内膜异位腺上皮不典型增生患者(不典型增生组)和30例卵巢子宫内膜异位症患者(异位症组)手术切除病灶中P16、P53、PTEN和hMLH1蛋白的表达,比较3组的阳性表达率。结果:P53蛋白在异位症组、不典型增生组和癌变组的阳性表达率逐渐升高,而P16、PTEN和hMLH1蛋白的阳性表达率则逐渐降低;在癌变组中,P16与PTEN和hMLH1蛋白的阳性表达率以及PTEN和hMLH1蛋白的阳性表达率呈正相关(r=0.375,P<0.05;r=0.527,P<0.01;r=0.425,P<0.05)。P53与P16、PTEN和hMLH1蛋白的阳性表达率呈负相关(r=-0.412,P<0.05;r=-0.572,P<0.01;r=-0.510,P<0.01)。结论:P53、P16、PTEN和hMLH1蛋白在卵巢子宫内膜异位症癌变中存在异常表达,4种蛋白的异常表达可能与卵巢子宫内膜异位症癌变的发生、发展有密切关系。

Objective： To investigate the expression pattern of P16, P53, PTEN and hMLH1 and roles they played in the malignant transformation of ovarian endometriosis. Methods： The protein expression of P16, P53, PTEN and hMLH1 in 32 cases of carcinoma arising in ovarian endometriosis （ carcinoma group） , and 17 eases of atypical hyperplasia of ovarian ectopic endometrial glands （ atypical hyperplasia group） as well as 30 eases of ovarian endometriosis （endometriosis group） were all examined by immunohistochemistry, The positive rates of these groups were compared. Results： The positive rate of P53 expression presented an increased trend from endometriosis group, atypical hyperplasia group to carcinoma group, while PI6, PTEN and hMLH1 showed a decreased trend. In carcinoma group, expressions of PTEN and hMLH1 were positively correlated with that of P16 （r = 0. 375, P 〈0. 05; r =0. 527, P 〈0. 01 ）, and expressions of PTEN and hMLH1 were also positively correlated with each other （r = 0. 425, P 〈0.05）. The expressions of P16, PTEN and hMLH1 all showed negative corelations with that of P53 （r = - 0. 412, P 〈 0. 05 ; r = - 0. 572, P 〈 0. 01 ; r = - 0. 510, P 〈 0. 01 ）. Conclusion： Protein expressions of P16, PTEN and hMLH1 were aberrant in ovrian carcinoma and they may play certain roles in malignant transformation of ovarian endometriosis.