不同来源白首乌rDNA ITS序列分析

Sequence analysis of rDNA-ITS of Bai Shouwu from different species

目的:研究白首乌不同种的ITS序列遗传差异性,分析序列差异性在白首乌品种鉴定及种质资源研究中的意义。方法:从不同来源的白首乌中提取总DNA,以核基因组ITS通用引物进行扩增,扩增产物经克隆后,进行测序。结果:获得4个不同来源样品的ITS全序列及5.8 S全序列以及两端的18 S,26 S的部分序列,其中戟叶牛皮消CynanchumbungeiITS序列为国际首次获得,登录号分别分别为GU198970(野生种),GU479037(栽培种)。各样品间ITS序列存在不同,4个样品序列间共存在10个变异位点。结论:ITS序列对白首乌种质资源鉴定具有较好的分辨性,为鉴别不同来源的白首乌提供了依据。

Objective： To study the genetic diversity of rDNA ITS sequences in different species of Bai Shouwu,utilize the molecular diversity of ITS sequences to authenticate the different species of Bai Shouwu.Method： Firstly,total DNA was extracted from the different species of Bai Shouwu.Secondly,the ITS sequence was amplified by PCR with universal primer of ITS and sequenced after cloning and purification.Result： From four species the complete sequence of ITS and 5.8 S rDNA,the partial sequences of 18S rDNA and 26S rDNA were obtained.The rDNA ITS sequences of Cynanchum bungei（sign in No.GU198970 and No.GU479037） were obtained.Ten variable sites among the sequences were found.Conclusion： ITS sequence could be used to authenticate the species.The method could be used to identify germplasm resources and authenticate.