组织因子途径抑制物-2在非小细胞肺癌中的表达及其与细胞凋亡的关系

TFPI-2 expression in non-small cell lung cancer and apoptosis

目的:探讨非小细胞肺癌中组织因子途径抑制物-2(TFPI-2)的表达及其与细胞凋亡的关系,评价其与肺癌恶性程度的关系。方法:收集非小细胞肺癌石蜡标本、临床资料和临床分期,采用免疫组化法检测TFPI-2蛋白在60例非小细胞肺癌和15例正常肺组织中的表达水平,并应用末端脱氧核苷酸转移酶介导的标记染色法(TUNEL法)检测细胞凋亡,计算凋亡指数(AI)。结果:TFPI-2在非小细胞肺癌中的表达指数为60.0%,低于正常肺组织的89.5%。Ⅰ、Ⅱ期及无转移的非小细胞肺癌组织中TFPI-2表达指数(70.9%)及AI值[(9.58±4.52)%]均高于Ⅲ、Ⅳ期[48.3%、(6.36±1.98)%]及有转移的非小细胞肺癌组织[57.1%、(6.10±2.68)%],差异有统计学意义(P<0.05)。结论:TFPI-2在正常肺组织中的表达水平明显高于非小细胞肺癌组织,TFPI-2可能在诱导非小细胞凋亡中起到重要作用。

Objective To investigate the relationship between tissue factor pathway inhibitor-2（TFPI-2） expression and apoptosis in non-small cell lung cancer（NSCLC）, and to evaluate the association of TFPI-2 with the malignancy degree of lung cancer.Methods Paraffin samples of NSCLC and clinical data and staging were collected.The expression levels of TFPI-2 protein were detected by immunohistochemistry in 60 patients and 15 healthy subjects.Apoptosis was detected by TUNEL assay and apoptotic index（AI） was calculated.Results The expression index of TFPI-2 was lower in NSCLC than in normal lung tissues（60.0% vs.89.5%）;The expression index of TFPI-2 and AI were significantly higher in stageⅠtoⅡdisease or non-metastatic disease than in stage Ⅲ to Ⅳ or metastatic disease [70.9% vs.48.3% and（9.58 ± 4.52）% vs.（6.36 ± 1.98）%, 70.9% vs.57.1% and（9.58 ± 4.52）% vs.（6.10 ± 2.68）%;P 0.05].Conclusions The expression level of TFPI-2 is significantly higher in normal lung tissues than in NSCLC.TFPI-2 may play an important role in inducing apoptosis in NSCLC.