转基因甘蔗对根际土壤微生物多样性的影响

Effect of transgenic sugarcane on microbial community diversity in rhizosphere soil

应用Biolog法研究了转几丁质酶和β-1,3-葡聚糖酶基因甘蔗对根际土壤微生物多样性的影响。结果表明：转基因甘蔗根际土壤微生物利用单一碳源能力（AWCD）要高于非转基因甘蔗的根际土壤微生物；转基因甘蔗和非转基因甘蔗根际土壤样品的Simpson指数（1/D）、Shannon-Wiener指数（H）、Mclntosh指数（U）均存在差异，其中转基因甘蔗根际土壤微生物的Simpson指数显著高于非转基因甘蔗土壤微生物，两种土壤微生物群落优势度的差别很大，其次是Mclntosh指数（U）指数，丰富度的差别不是很大。说明转基因甘蔗根系在生长期的分泌物刺激根际土壤微生物群落的生长，可能诱导了具有某些特定生理特征的微生物群落的发展，因此转基因甘蔗对根际土壤微生态有一定的影响。

Effect of chitinase and β-1,3- glucanase transgenic sugarcane on microbial diversity in rhizosphere soil was studied by Biolog plates method. The results showed that the Average Well Color Development （AWCD） of transgenic sugarcane was higher than non-transgenic sugarcane, indicating the carbon utilization capability of microbial community was higher. The indices of Shannon （H）, Smipson （1/D） and McIntosh （U） were calculated respectively to show the richness, dominance and evenness of the functional diversity accordingly. The results showed that the rhizosphere soil microbial diversity indices of transgenic sugarcane were all higher than those of non-transgenic sugarcane; the dominance of the functional diversity was remarkably improved, followed by the evenness; the difference of the richness was not significant. It indicated that the root exudates of transgenic sugarcane in the growing season had stimulated the growth of rhizosphere soil microbial, which might have induced the development of the microbial community with certain physical characteristics. This preliminary study showed transgenic sugarcane might affect the microbial community composition in rhizosphere soil.