病毒性肝炎临床诊治的基础与应用研究

Basis and Applied Study of Clinical Diagnosis and Treatment on Viral Hepatitis

病毒性肝炎是我国最重要的传染病之一，可导致严重慢性肝病，并且与肝细胞癌密切相关。近５年来，我们改进肝炎病毒核酸诊断技术，建立热变性ＨＣＶＲＮＡ直接扩增技术、ＲＴＰＣＲ夹心斑点杂交技术、ＨＢＶ前Ｃ区点突变检测技术，以及克隆ＨＣＶ、ＨＧＶ基因序列。此外，对广东甲型肝炎流行病学调查与输血传播ＨＣＶ、ＨＧＶ及ＴＴＶ人群感染的情况、慢性ＨＢＶ感染者病情反复发作的因素、ＨＣＶ与肝癌关系、干扰素、Ｌａｍｉｖｕｄｉｎｅ的临床治疗观察、基因治疗的应用基础研究，以及中西医结合治疗病毒性肝炎等作了一系列研究。这些技术的改进与研究成果，迅速应用到临床防治工作中，对当前病毒性肝炎诊治工作起到积极的推动作用。

Viral hepatitis is one of the most important infectious diseases in China. It is the most common cause of chronic hepatitis and is closely related to hepatocellular carcinoma. In the past 5 years, we have improved the molecular biology techniques for the diagnosis of this group of diseases, including the establishment of direct amplified heat denatured detection of HCV RNA, detection of pre core region mutant of HBV with RT PCR, dot hybridization and the sequence cloning of HCV and HGV. A series of epidemiologic studies including the investigation of epidemiology of HAV in Guangdong, as well as risk factors study on transmission of HCV, HGV and acute exacerbation in chronic hepatitis B patients was carried out. Preliminary basic study on gene therapy and a series of studies on combination of Chinese traditional medicine with western medicine are also in progress. All these achievements have been rapidly applied to clinical practice and played important roles in the advance of diagnosis and treatment in viral hepatitis.