恙虫病立克次体型特异性基因序列的扩增与鉴定

Aplification and Identification of the Gene Encoding the Typespecific Antigen from Rickettsia Tsutsugamushi

目的：明确广东地区恙虫病立克次体（Ｒｔ）的流行株型别。方法：以Ｒｔ主要外膜蛋白５６ｋＤａ型特异抗原基因（ｔｓａ５６Ｋｄａ）为靶基因设计并合成群、型特异引物，采用ＮＰＣＲ法对广东地区的标本进行株型鉴定。结果：广东顺德、南海等地恙螨、野鼠及患者所携带Ｒｔ为Ｋａｒｐ株。结论：顺德、南海等地流行优势株为Ｋａｒｐ株，且媒介恙螨。

Objective: To define the type of Rt in endemic areas of tsutsugamushi disease of Guangdong. Methods: The group and types primers were derived from 56 kDa protein of Rt. Nested polymerase chain reaction (NPCR) method was used for detecting and typing Rickettsia tsutsugamushi (Rt). Then the strain specific gene sequence of local epidemic strains were cloned. Results: The type of Rt in specimens from Shunde and Nanhai were Karp strain and the positive recombinant pUC18 RK was conducted. Conclusions: The main epidemic strain in Shunde and Nanhai is Karp strain and the vector chigger mites, wild rats and patients carry the same type of Rt.