胰岛素对大鼠局灶缺血脑组织c-fos基因表达的影响

Insulin Effect on c fos Gene Expression in Focal Ischemic Rat Brain

目的：观察胰岛素对局灶缺血脑组织ｃｆｏｓ基因表达的影响。方法：３０只肾血管性高血压大鼠（ＲＨＲ）随机分４组，在大脑中动脉闭塞（ＭＣＡＯ）后即予胰岛素（２．１ＩＵ／ｋｇ，Ａ组）、葡萄糖（２ｇ／ｋｇ）和低剂量胰岛素（２．１ＩＵ／ｋｇ，Ｂ组）、葡萄糖（４ｇ／ｋｇ）和高剂量胰岛素（４．５ＩＵ／ｋｇ，Ｃ组）、生理盐水（Ｄ组），采用原位杂交技术检测各组ＭＣＡＯ后３ｈ脑组织ｃｆｏｓ基因的表达。结果：低剂量胰岛素组（Ａ、Ｂ组）ｃｆｏｓｍＲＮＡ未出现明显变化（Ｐ＞００５），而Ｃ组ｃｆｏｓｍＲＮＡ则有统计学意义的增加（Ｐ＜００１）。结论：胰岛素促进缺血脑组织ｃｆｏｓ基因表达，可能是其神经保护作用机制之一。

Objective: To investigate the effect of insulin on c fos gene expression in focal ischemic rat brain. Methods: The renovascular hypertensive rats(RHR) (n=30) were divided at random into 4 groups which received separately with insulin only (group A, 2.1IU/kg),insulin and glucose (group B with low dose insulin, 2.1IU/kg; group C with high dose insulin, 4.5IU/kg) or normal saline (group D) immediately after middle cerebral artery occlusion (MCAO). Level of c fos mRNA on brain sections was measured by in situ hybridization (ISH) at 3h after MCAO. Results:Little effect on c fos expression was detected in groups with low dose of insulin (group A, B) (P>0.05) and a significant increase was detected in group C(P<0.01). Conclusions: These results indicate that the effect of insulin on c fos expression may be one of the mechanisms of neuroprotection of insulin.