摘要
目的:探讨广州地区丙型肝炎病毒(HCV)基因型分布的特点以及HCV基因型与临床病情的关系。方法:病例选自广州居民,基因分型使用Okamoto的“型特异性引物PCR法”,并加以改进,基因型的命名采用国际上统一的Simmonds两级命名体系。结果:广州HCV基因型分布1a占76%、1b占495%、2a占238%、2b占19%、3a占29%、1b+2a混合型占95%、未定型占5%,同国内其它地区一些学者所报道的结果相似。结论:HCV基因型分布不存在先前认为的地理上的南北差异。不同基因型传播途径没有差异,均以血液传播为主(829%)。1型及混合型感染病情较重。
Objective: The distribution of HCV genotypes in Guangzhou and the relationships between HCV genotypes and clinical features were studied. Cases were selected from the patients hospitalized in our department from 1994 to 1997. All of them are Guangzhou residents. Methods: HCV RNA and genotypes were detected by RT PCR with a pair of primers according to the sequence of 5′ NCR and with genotyping primers according to the sequence of C regionoriginally proposed by Okamotot et al. Results: 8(7.6%) of 105 patients with hepatitis C in Guangzhou were identified as 1a, 52(49.5%) were 1b, 25(23.8%) were 2a, 2(1.9%) were 2b, 3(2.9%) were 3a, 10(9.5%) were 1b+2a and 5(5.0%) were unidentified. The distribution of HCV genotypes is similar to those reported by other authors in China, with 1b as the predominant type, followed by 2a and other genotypes. Conclusions: The distribution of HCV genotypes has no geographical distinction between norhtem China and southern China previously proposed by some researchers. There is no evident relationship between HCV genotypes and different transmission routes. Blood transfusion was found to be the major transmission route (82.9%) for all HCV genotypes. Infection with 1b or 1b+2a tends to lead a more severe liver disease and poorer response to interferon therapy.
出处
《中山医科大学学报》
CSCD
1999年第1期69-72,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
关键词
丙型肝炎
遗传学
基因分型
PCR
干扰素
药物疗法
hepatitis C/genetics
genotype
polymerase chain reaction
interferons/therapeutic use