多巴胺转运体放射自显影对帕金森病的初研

The Preliminary Study of Dopamine Transportor Autoradiography in Parkinson′s Disease

目的：多巴胺转运体（ＤＡＴ）是位于多巴胺神经元突触前膜的糖蛋白分子，在多巴胺的回收中起着重要作用，与帕金森病密切相关。为进行βＣＩＴ的核素显像检查打基础，我们进行了本课题研究。方法：用１２５ＩβＣＩＴ作示踪剂，进行大鼠脑ＤＴＡ的放射自显影及时间放射性分布研究。结果：１２５ＩβＣＩＴ能成功地分布到大脑纹状体部位；时间放射性分布图象显示纹状体部位与大脑颞叶皮质（靶／非靶）光密度比值的最高值出现在静脉注射示踪剂后２０ｈ左右。结论：βＣＩＴ是一种有潜力的ＤＡＴ显像剂，其显像的最佳时间在注射后２０ｈ左右。

Objective: Dopamine transportor (DAT) is a glycoprotein situated in the presynaptic membrane of Dopamine neuron.It plays a important role in re uptake of the endogenous Dopamine,and closely relates to the Parkinson′s disease.This expreiment studied DAT distribution in the brain of rat. Methods: DAT and its Time distribution in the brain of rat were studied by an autoradiographic method using 125 I β CIT as a tracer. Results: This experiment showed the 125 I β CIT can be effectively concentrated in the striatum.Time distribution study demonstrated that the maximum striatum/cerebrum ratio is at 20 h after i.v. Conclusions: β CIT is a potential imaging agent of Parkinson′s disease,the best imaging time is about 20 h after i.v..This experiment gives a good basis for 123 I β CIT image and the early diagnosis of Parkinson′s disease.