急性下呼吸道感染时H ib OMP特异抗体测定的意义

Hib OMP specific antibody assays in the diagnosis of acute lower respirtory tract infection in children

我们建立了ｂ 型流感嗜血杆菌（Ｈｉｂ）外膜蛋白（ＯＭＰ）特异抗体测定的间接酶联免疫吸附法（ＥＬＩＳＡ），同时联合血、尿细菌抗原检测，对７０ 例肺炎患儿及３０ 例健康儿童进行研究。结果有１９／７０（２７．１％ 例）肺炎患儿产生Ｈｉｂ ＯＭＰ抗体反应，除外１ 例交叉反应，余１８ 例诊断为Ｈｉｂ 感染，其中５ 例未查到Ｈｉｂ 抗原，以Ｈｉｂ ＯＭＰ特异抗体反应得到诊断。表明Ｈｉｂ ＯＭＰ抗体测定可提高小儿Ｈｉｂ 肺炎的病原诊断率。

An indirect enzyme linked absorbent assays (ELISA) to measure the antibody against haemophilus influenzae type b (Hib) outer menbrane protions (OMP) was established and combined with antigen detection from blood and urine. 70 cases of pneumonia and 30 cases of healthy control were investigated. The results showed that antibody response were observed in 19 (27 1%) of the 70 children with pneumonia. Excluding one case of cross\|reaction, the others 18 cases, were diagnosed as Hib infection five caese had no Hib antigen evidence. Their diagnosis were confirmed only by the antibody response. The results suggest that the assay of antibody to Hib OMP can elevate the rate of the pathological diagnosis of Hib pneumonia.\;