反相高效液相色谱法测定化妆品中性激素

Determination of sexual hormones in cosmetics by using Re HPLC method

采用反相高效液相色谱法测定化妆品中雌三醇等７种性激素，以ＺｏｒｂａｘＣ１８为固定相，甲醇－水（８０＋２０）为流动相，检测波长为２５４ｎｍ，样品用甲醇超声提取，回收率为８６．６％～１０４．４％，相对标准偏差为１．２％～５．８％。

A Re HPLC method for the determination of seven kinds of sexual hormones, such as estriol in cosmetics was established. Zorbax C 18 and Methanol water were used as solid phase and liquid phase respectively. Methanol was used to extract the hormones in samples using a ultrasonic method. In the background with cosmetic samples, the relative standard deviations of those seven kinds of added sexual hormones were 1 2%～4 4% at low concentration and 2 1%～5 8% at high concentration. The recoveries rates of those hormones were 87 7%～103 1% and 86 6%～104 4%, respectively.