调理脾胃复方对PTZ点燃癫痫模型阿片肽含量的影响

The Effects of Spleen and Stomach Regulating Prescriptions on Opioid Peptide Concentration in PTZ Kindled Rats

为了研究调理脾胃复方的抗痫作用，本实验采用放免法观察大鼠ＰＴＺ点燃以及服用调理脾胃复方治疗后，脑内阿片肽含量的变化。结果表明，ＰＴＺ点燃大鼠海马内亮脑啡肽（ＬＥＮＫ）、β内啡肽（βＥＰ）明显升高，强啡肽Ａ１－１３（ＤＹＮＡ１－１３）显著降低；大脑皮层内ＬＥＮＫ明显升高，ＤＹＮＡ１－１３、β－ＥＰ无明显变化。用调理脾胃复方治疗后，上述脑区ＬＥＮＫ、βＥＰ显著减少，ＤＹＮＡ１－１３明显增加。提示癫痫发作及调理脾胃复方抗痫作用可能与脑内ＬＥＮＫ、βＥＰ、ＤＹＮＡ１－１３的变化有关。

Radioimmunologic method was used to evaluate the influence of the complex prescription of regulating the spleen and stomach on the pentylenetetrazole (PTZ) kindled rats and changes of the opioid peptides concentration in the brain. In the hippocampus of the PTZ kindled rats, the leucine-enkephalin (L ENK) and β endorphin (β EP) were increased and dynorphin A1-13 (DYN A1-13 ) decreased. After treated with the complex prescription, the L ENK andβ EP were markedly decreased, while DYN A1-13 increased. It indicates that the epileptic attack and the antiepileptic effect of the complex prescription may relate with the changes of the L ENK、β Ep and DYN A1-13 in the brain.