天麻素对谷氨酸致培养皮层神经细胞损伤的保护作用

Protective Effects of Gastrodine on Injured Cortical Neurons by Glutamic Acid

取新生大鼠大脑皮层进行体外神经细胞培养，用谷氨酸建立离体的神经元损害模型，观察天麻素对兴奋性氨基酸神经毒性的影响。结果表明：２００μｍｏｌ／Ｌ谷氨酸作用１０ｍｉｎ能造成培养神经元的大量死亡，培养液中乳酸脱氢酶（ＬＤＨ）含量明显增高；在培养液中加入天麻素或氯胺酮可明显降低神经细胞死亡率，减少乳酸脱氢酶的漏出。提示天麻素可拮抗兴奋性氨基酸神经毒性。

Cerebral cortex of the newborn rat was used for neurons culture in vitro. An isolated pattern of injured neuro by glutamic acid was built up. Observe the effect of gastrodine on the neurotoxicity of excitatory amino acid. The results show that: Glutamic acid can cause the expiration of cultured neurons in large quantities and the amount of lactic dehydrogenase (LDH) in the nutrient solution can be markedly increased. By adding gastrodine or ketamine into the nutrient solution, mortality rate of the neurons can be significantly decreased and the transudation of LDH also diminished, which suggests gastrodine can antagonize the neurotoxicity of excitatory amino acid.