胃癌血清CK-M_2、寡糖蛋白测定的临床意义

Assessment the Clinical Value of CK-M 2 and Oligosaccharide Protein in Serum from Patients with Gastric Carcinoma

本文采用琼脂糖电泳法测定血清磷酸肌酸激酶大分子同工酶－２（ＣｒｅａｔｉｎｅＫｉｎａｓｅｉｓｏｅｎｚｙｍｅ２，ＣＫ－Ｍ２）和酶免疫双抗体夹心法测定可溶性寡糖蛋白（ＯｌｉｇｏＭａｃｒｏｓａｃｃｈａｒｉｄｅＰｒｏｔｅｉｎ，ＯＰ）含量。经５７例胃癌、５１例胃癌前期病变（慢性萎缩性胃炎１９例，慢性萎缩性胃炎伴肠化１７例，残胃炎１１例，不典型增生４例）和２８例正常献血员血清测定。结果表明正常人血清ＣＫ－Ｍ２均为阴性。胃癌检出阳性率为５６１％，在胃部疾患中特异性为９８６３％，ＯＰ在胃癌组明显高于其他两组（Ｐ＜００５）。但ＣＫ－Ｍ２、ＯＰ与胃癌细胞类型及分化程度无关。提示ＣＫ－Ｍ２可作为胃癌的辅助诊断指标，尤其在胃部疾患的鉴别诊断上具有一定的意义；ＯＰ可作为评估胃癌患者体内免疫活性的指标。

Objective:To evaluate the clinical value of creatine kinase macro isoenzyme type 2(CK-m 2) and Oligosaccharide protein (OP) in serum from patients with gastric carcinoma (GC).Methods:Serum level of CK-m 2 was detected by agar gel electrophoresis.OP concentration was measured by an enzyme immunoassay.Results:Serum levels of CK-m 2 and OP in 57 cases of GC were significantly higher than those in 51 cases with gastric precancerous lesion and 28 controls.The diagnostic sensitivity and specificity for GC with CK-m 2 was 56 10% and 98 63%,respectively.CK-m 2 and OP werent associated with histologic type and degree of differentiation.Conclusion:These results suggest that CK-m 2 may serve as a mark to diagnose GC,and the specificity is higher.Whereas,OP isnt more significant for GC diagnosis,but it could be a useful indicator for evaluating the status of body immune.