血吸虫重组BCG-Sj26GST疫苗接种后保护力的观察

Observation on Protection by Immunization with Recombinant BCG Sj26GST Vaccine of Schistosome

为了探讨血吸虫重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗免疫鼠后对尾蚴攻击感染的保护性作用，采用１０６ＣＦＵ和１０８ＣＦＵ重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗皮下接种ＢＡＬＢ／Ｃ鼠，接种后８周用日本血吸虫尾蚴进行攻击感染，感染后６周剖杀小鼠，计算减虫率、减卵率，同时进行虫卵肉芽肿周长和面积测量，以及抗体水平和抗原水平测定，同时设有ＰＢＳ对照组。结果表明重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗免疫小鼠后，１０６ＣＦＵ、１０８ＣＦＵ组与对照组相比，减虫率分别为２７．３４％、１６．６４％，减卵率分别为５５．７５％、６０．５０％，虫卵肉芽肿的平均周长和平均面积、免疫后血清抗体水平差别均具有非常显著意义；１０８ＣＦＵ与１０６ＣＦＵ组相比，血清抗原水平差别具有显著意义，说明血吸虫重组ＢＣＧ－Ｓｊ２６ＧＳＴ疫苗是一种比较理想的新型疫苗。

In order to investigate the protection against challenge with cercarie of schistosoma japonicum, by immunization with recombinant BCG Sj26GST vaccine, BALB/C mice were immunized subcutaneously by 10 6CFU and 10 8CFU rBCG Sj26GST vaccine of schistosome respectively, challenged with Sj cercarie on the 8th week of inoculation and killed on the 6th week of infection to count rate of reduced worm burdens, rate of reduced eggs number, to measure the circumference and the area of egg granulomas,and to determine the level of antibody and circulating antigen. At the same time, PBS served as control group. The results showed that after the mice were immunized with the vaccines, the rate of reduced worm burdens and the rate of reduced eggs number were 27 36 % vs 16 64 % and 55 75 % vs 60 50 % respectively in the experimental group and control groups, and average circumference, average area and the level of antibody had a significant difference between the groups. The level of circulating antigen showed a marked difference between 10 8 CFU and 10 6 CFU groups. It is suggested that rBCG Sj26GST vaccine of schistosome is a new type of more ideal vaccine.