应激对T淋巴细胞增殖的抑制作用

SUPPRESSIVE EFFECT OF RESTRAINT STRESS ON T-LYMPHOCYTE PROLIFERATION

使用束缚方法使大鼠产生应激，一部分实验取大鼠应激３ｈ、６ｈ、１２ｈ和１８ｈ的淋巴结提取物，与刀豆素Ａ（ＣｏｎＡ）同时加入到正常大鼠肠系膜淋巴结细胞悬液中，浓度比为１∶１６０和１∶６４０。另一部分实验取大鼠应激３ｈ、６ｈ、１２ｈ、１８ｈ和解除束缚后１２ｈ、２４ｈ、４８ｈ、７２ｈ、９６ｈ的血清，与ＣｏｎＡ同时加入到正常大鼠肠系膜淋巴结细胞悬液中，浓度比为１∶１６和１∶６４，孵育６８ｈ后用噻唑蓝（ＭＴＴ）比色分析法检测肠系膜淋巴结Ｔ细胞增殖的光密度（ＯＤ）值。旨在说明应激抑制Ｔ细胞增殖的出现、加强和恢复过程。结果如下：（１）应激３ｈ和６ｈ大鼠的淋巴结提取物和血清对Ｔ细胞的增殖都没有明显的影响。（２）应激１２ｈ，两种浓度的淋巴结提取物和血清都出现对Ｔ细胞增殖的明显抑制作用，应激１８ｈ的作用更强。（３）束缚解除后，血清对淋巴细胞的抑制作用仍然存在，但随着时间的延长抑制作用逐渐减弱，至束缚解除后９６ｈ，抑制作用基本消失。上述结果提示，大鼠束缚应激能明显抑制Ｔ淋巴细胞的增殖；这种抑制作用需要束缚应激一段时间后才能出现；并且在解除束缚后仍能持续较长时间。

In the present study, restraint, which causes no direct tissue damage, was selected as a stressor to investigate the suppressive effect of the restraint stress on the T-lymphocyte proliferation in rats. The extract from the lymph node, which was respectively drawn at 3 hour, 6 hour, 12 hour, 18 hour of restraint stress and the serum, which was respectively drawn at 3 hour, 6 hour, 12 hour, 18 hour of restraint stress and at 12 hour, 24 hour, 48 hour, 72 hour, 96 hour after the stress, were added to the lymphocyte suspension of mesenteric lymphaden of rat with concanavalin A (Con A) and were incubated for 68 hours. The optical density (OD) values, which show the level of T-lymphocyte proliferation, were determined by means of the colorimetric assay of methylthiazol tetrazolium bromide (MTT). The results are as follows:(1)The extract from the lymph node and the serum drawn at 3h and 6h of stress did not markedly affect the T-lymphocyte proliferation.(2)At 12h of stress, the extract from the lymph node and the serum significantly inhibited the lymphocyte transformation, the inhibition being stronger at 18h of stress.(3)After the restraint stress, the suppressive effect of serum on the T-lymphocyte proliferation gradually decreased, and to 96h after the stress, the suppression basically disappeared.The above results suggest that restraint stress may cause the inhibition of T-lymphocyte proliferation, and it takes some time for the inhibitory effect to appear and tonger time to disappear.