摘要
目的探讨肥胖大鼠不同病程中血清白细胞介素-18(IL-18)水平的变化及其与胰岛素抵抗的关系。方法将36只雄性SD大鼠随机分成肥胖组和对照组,分别于第14及20周末处死大鼠收集血清标本,检测各组大鼠血清IL-18、IL-6、肿瘤坏死因子-α(TNF-α)和C反应蛋白(CRP)水平。结果第14和20周末,肥胖组大鼠血清IL-18、IL-6、TNF-α和CRP水平均显著高于相同周数对照组大鼠(P<0.01)。但不同周数肥胖组大鼠血清IL-18、IL-6、TNF-α和CRP水平比较差异无统计学意义(P>0.05)。Pearson直线相关分析显示,第14和20周末,肥胖组大鼠血清IL-18均分别与体重、内脏脂肪重量和CRP呈正相关性,与胰岛素敏感指数(ISI)呈负相关。结论不同病程中,肥胖大鼠血清中IL-18等炎症因子水平无明显变化,IL-18与肥胖及其相关的胰岛素抵抗密切相关。
Objective To investigate the changes of serum interleukin-18(IL-18)level in different course and its associativity with insulin resistance in obese rats.Methods 36 Sprague-Dawlay male rats were randomly divided into two groups:the obese group and the control group.The blood sample was collected when the rats were killed at the end of the 14th and 20th week.The levels of serum IL-18,IL-6,tumor necrosis factor-α(TNF-α)and C-reactive protein(CRP)were assayed.Results Both at the end of the 14th week and at the end of the 20th week,the levels of serum IL-18,IL-6,TNF-α and CRP were significantly higher in obese group than those in control group with the same week(P〈0.01).But in different week,the levels of serum IL-18,IL-6,TNF-α and CRP had no statistic significance in obese group(P〉0.05).Pearson linear correlation analysis showed that the level of serum IL-18 in obese group was positive correlation with body weight,viscera adipo weight and CRP,and inverse correlation with insulin sensitivity index(ISI).Conclusion The levels of inflammatory factors such as interleukin-18 did not change significantly in different course,interleukin-18 played an important role in obesity and insulin resistance related to obesity.
出处
《中华全科医学》
2010年第7期811-812,共2页
Chinese Journal of General Practice